MULTIDISCIPLINARY ANALYSIS OF HIV-1 ELITE

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Sample loading via tubes. The BD LSR Fortessa is equipped with 5 lasers (355, 405, 488, 561,640 nm). It allows detection of up to 18 parameters simultaneously, 16 fluorescent channels plus forward and side scatter. See configuration. For more information about the LSR Fortessa please visit the Becton Dickinson website or contact dirk.pacholsky@igp.uu.se. FACS Diva quick BD LSRFortessaを用い、健康なドナーの全血をBD Pharm Lyse™ lysing bufferを用いて溶血し、T細胞のサブセットを解析しました。データは、Blue、Red、Violetのレーザーで励起した10カラーのパネルを示します。 BD LSR Fortessa Flow Cytometry Analyzer equipped with HTS (High Throughput Sampler) The facility at 2117A Everitt Lab (click link for campus map) is equipped with a BD LSR Fortessa analyser. It is very similar in operation to the LSR II cytometers (RAL and ERML Satelite Facilities) but with greater analysing sensitivity.

eosinofiler & neutrofiler) karaktäriseras med flödescytometri (BD™ LSR Fortessa™. 4-Laser Flow Cytometer). Extracellulär immunsignalering (IL-26 & andra IL-. Cells were analyzed using FACSCalibur, or LSR II Fortessa cytometers and CellQuestPro or DIVA softwares (BD Bioscience). Cell transfer. CD4 + spleen T cells All monoclonal antibodies were obtained from BD PharMingen. After washing, cells were analyzed using LSRII Fortessa™ cell analyzer (BD Biosciences, CA), Poland Manufacturas Anframa, S.A Olias del Rey (Toledo), Spain 8.0 B D J55 Kupferflaschen München, 11.0 F60 Fortessa Tableware Solutions Ashburn, Fisher Scientific and Jackson ImmunoResearch, 1:500). Cells were analysed on an LSR Fortessa and sorted with a BD Influx Cell Sorter (BD Biosciences).

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Location: 300 George Street, rooms 345 and 345A. Contact: Lesley.Devine@yale.edu. The Fortessas are within rooms that are designated BSL2 space to FORTESSA USER GUIDE COMPUTER SETUP: • Turn on computer before turning on the machine • Windows 10 Username: BDOperator • Password: BDIS#1 • Open Tera Term application on desktop and leave running in background • Log in to BD FACSDiva software MACHINE SETUP: • Turn on the machine if you are the first user of the day.

MULTIDISCIPLINARY ANALYSIS OF HIV-1 ELITE

Designed for customization and optimized for reliability, the BD LSRFortessa family of cell analyzers allow you freedom of choice to achieve your research goals–your way.

BD LSRFortessa™ (2) Sort by: Instrument Systems (2 of 2) results found.
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Flow Cytometry Shared Resource. BD Fortessa Configuration. KCRB.
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Fours lasers: 407 nm, 488 nm, 561nm, and BD Fortessa X20 A Configuration and Fluorochromes Laser Diva Parameter Name Fluorochromes 488 nm Blue B 530/30 BB515, FITC, AF488, CFSE, GFP, TO-PRO-1 BD FACS LSR Fortessa User Manual Flow Cytometry Unit – Biomedical Core facility Technion Faculty of Medicine For help please call: Amir Grau 054-210-0778 Yaakov Sakoury 050-749-8565 Ofer Shenker 052-456-8846 A. Starting Up 1. Turn on the instrument: a. LSR Fortessa - red switch on the wall behind the monitors (Fig.

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Extracellulär immunsignalering (IL-26 & andra IL-. Cells were analyzed using FACSCalibur, or LSR II Fortessa cytometers and CellQuestPro or DIVA softwares (BD Bioscience). Cell transfer. CD4 + spleen T cells All monoclonal antibodies were obtained from BD PharMingen. After washing, cells were analyzed using LSRII Fortessa™ cell analyzer (BD Biosciences, CA), Poland Manufacturas Anframa, S.A Olias del Rey (Toledo), Spain 8.0 B D J55 Kupferflaschen München, 11.0 F60 Fortessa Tableware Solutions Ashburn, Fisher Scientific and Jackson ImmunoResearch, 1:500). Cells were analysed on an LSR Fortessa and sorted with a BD Influx Cell Sorter (BD Biosciences). BioVis Flödecytometri-noden består för närvarande av fem instrument, en BD erfarenhet av FACS- analys, gärna på plattformarna FACS Aria och Fortessa. BioVis Flödecytometri-noden består för närvarande av fem instrument, en BD erfarenhet av FACS- analys, gärna på plattformarna FACS Aria och Fortessa.